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Tubos para HPLC

HPLC Tubing

HPLC tubing, also known as capillary or chromatographic tubing, is a critical component of HPLC systems. It functions as a conduit for the mobile phase and sample, enabling their flow throughout the chromatographic setup.

HPLC tubing is characterized by its small diameter, typically ranging from 1 mm to 5 mm or smaller. The narrow diameter allows for efficient liquid flow and reduces system volume, minimizing sample dilution and enhancing separation resolution.

The length of HPLC tubing varies based on system complexity and requirements, spanning from a few centimetres to several meters. Longer tubing lengths can increase separation efficiency and provide more time for interaction between the sample and stationary phase.

The inner surface of HPLC tubing must be smooth and inert to minimize interactions with the sample. A smooth surface reduces the potential for sample adsorption or carryover between runs, ensuring accurate and reproducible results.

HPLC tubing is connected to various system components, including injectors, columns, and detectors, using fittings or connectors. Secure and leak-free connections are vital to maintain system integrity and prevent sample loss or contamination.

The choice of HPLC tubing depends on factors such as analyte properties, required flow rate, and compatibility with the mobile phase and solvents. Tubing materials must exhibit chemical resistance, low adsorption characteristics, and mechanical stability to withstand system pressures and temperatures.

HPLC tubing is available in various materials, each with its own considerations and advantages in terms of chemical compatibility, thermal stability, and mechanical strength.

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  • PEEK GF30

    PEEK GF30 refers to a grade of PEEK material that is reinforced with 30% glass fiber.

    PEEK GF30 sheets or plates have the same base PEEK polymer as PEEK 450G but with the added enhancement of glass fiber reinforcement. The glass fibers enhance the mechanical properties of PEEK, providing increased strength, stiffness, and dimensional stability.

    PEEK GF30 sheets retain the other desirable properties of PEEK, such as high-temperature resistance, chemical resistance, and low friction coefficient.

    These sheets are commonly used in applications where improved mechanical performance, wear resistance, and strength are required, such as automotive components, aerospace parts, electrical connectors, and industrial applications.

  • PEEK 450g

    PEEK 450G refers to a specific grade of PEEK material. The "450G" designation signifies a standard, unfilled grade of PEEK manufactured by Victrex. PEEK 450G sheets or plates are composed solely of PEEK polymer without any reinforcement materials like glass fibers or carbon fibers. These sheets offer the inherent properties of PEEK, including high temperature resistance, excellent mechanical strength, chemical resistance, low friction coefficient, and dimensional stability.

    PEEK 450G sheets are typically available in various thicknesses and sizes to suit different application requirements.

    They find applications in industries such as aerospace, automotive, electronics, medical, and more, where the outstanding properties of PEEK are beneficial.

  • We use these two types of PEEK due to the enhanced qualities they have over standard PEEK meaning that our tubing is more effective in the HPLC application. These Benefits are:

  • Enhanced Purity

    Victrex 450g PEEK undergoes stringent manufacturing processes and quality controls, resulting in higher purity levels compared to generic PEEK. This enhanced purity reduces the risk of impurities and contaminants, ensuring better performance and minimizing interference with samples or processes.

  • Improved Mechanical Properties

    Victrex 450g PEEK offers enhanced mechanical strength, stiffness, and toughness compared to standard PEEK. These improvements can result in better resistance to stress, cracking, and vibration, ensuring reliable performance in demanding applications.

  • Optimized Chemical Resistance

    Victrex 450g PEEK exhibits superior chemical resistance compared to generic PEEK. It may provide better resistance to a wide range of solvents, acids, bases, and other aggressive chemicals, ensuring long-term stability and reliability in harsh chemical environments.

  • Enhanced Dimensional Stability

    Victrex 450g PEEK offers improved dimensional stability, maintaining its shape and dimensions even under high temperatures or prolonged use. This enhanced stability contributes to tighter tolerances, better sealing, and improved overall performance in critical applications.

  • Tailored Formulation

    Victrex 450g PEEK has specific modifications or formulation adjustments to enhance certain properties based on targeted applications. These modifications could be related to thermal stability, wear resistance, electrical conductivity, or other performance parameters, resulting in a more optimized material for specific use cases.

What Is PEEK And Why Do We Use It In Our HPLC Tubing

PEEK (polyether ether ketone) is a high-performance thermoplastic polymer with excellent mechanical, thermal, and chemical properties. It is widely used in various industries, including HPLC, due to its desirable characteristics.

PEEK is employed in HPLC tubing primarily because of its chemical compatibility and inertness. As HPLC involves the separation and analysis of various compounds, it is essential to use tubing materials that do not react or interact with the sample components. PEEK exhibits exceptional resistance to a wide range of solvents, acids, bases, and other chemicals commonly used in HPLC. Its inert nature ensures that it does not introduce unwanted interactions or contaminations that could affect the accuracy or reliability of the analysis.

Additionally, PEEK tubing offers high mechanical strength and stability, allowing it to withstand the pressures and temperatures experienced during HPLC analysis. It maintains its dimensional integrity under the operational conditions of the system, ensuring consistent flow and reliable results.

PEEK also possesses good thermal stability, enabling its use in HPLC systems that operate at elevated temperatures. This property is particularly beneficial in applications such as reversed-phase chromatography, where higher temperatures can enhance separation efficiency. Furthermore, PEEK is a biocompatible material, making it suitable for use in HPLC applications involving biological samples or biomolecules.

We use two different types of PEEK for our tubing which are:

Los tubos para HPLC fabricados con polieteretercetona (PEEK) son una opción popular en los sistemas de cromatografía líquida de alto rendimiento (HPLC) debido a su excelente resistencia química, alta resistencia mecánica y propiedades de baja extractabilidad. El PEEK es un polímero termoplástico que presenta una estabilidad excepcional en condiciones de alta presión y alta temperatura, lo que lo hace adecuado para aplicaciones exigentes de HPLC. A continuación, describiré y explicaré las características y ventajas de los tubos de PEEK en los sistemas de HPLC:

  1. Resistencia química: Los tubos PEEK son muy resistentes a una amplia gama de disolventes orgánicos, ácidos, bases y sales. Puede soportar fases móviles agresivas y mantener su integridad sin degradación ni lixiviación de contaminantes. Esta resistencia química garantiza la pureza y fiabilidad del sistema HPLC, evitando cualquier interacción no deseada entre el tubo y la muestra o la fase móvil.

  2. Alta resistencia mecánica: Los tubos de PEEK poseen excelentes propiedades mecánicas, como una elevada resistencia a la tracción y a la tensión y los impactos. Puede soportar las altas presiones típicas de los sistemas HPLC sin distorsión ni fallos. La robustez de los tubos de PEEK garantiza un rendimiento fiable y constante, incluso en las condiciones de funcionamiento más exigentes.

  3. Bajo volumen muerto: El tubo PEEK tiene un diámetro interno pequeño, lo que se traduce en un bajo volumen muerto. El volumen muerto se refiere al volumen de líquido retenido dentro del tubo y los accesorios, que puede causar ensanchamiento de banda y afectar a la resolución cromatográfica. El bajo volumen muerto de los tubos PEEK minimiza la dispersión de la muestra y permite obtener picos más nítidos, mejorando el rendimiento cromatográfico del sistema HPLC.

  4. Inercia: El PEEK es un material altamente inerte, lo que significa que no reacciona con los analitos ni con los componentes de la fase móvil, ni los adsorbe. Esta inercia es crucial para un análisis por HPLC preciso y reproducible, ya que evita interacciones no deseadas o pérdidas de analitos durante el proceso de separación. Los tubos de PEEK garantizan una adsorción mínima de la muestra, asegurando la integridad y fiabilidad de los análisis cuantitativos y cualitativos.

  5. Biocompatibilidad: El PEEK es biocompatible, por lo que es adecuado para aplicaciones de HPLC que impliquen el análisis de muestras biológicas, como péptidos, proteínas y compuestos farmacéuticos. No introduce contaminantes ni interfiere con los componentes de la muestra, preservando la integridad y fiabilidad de los ensayos bioanalíticos.

  6. Estabilidad térmica: Los tubos de PEEK presentan una excelente estabilidad térmica, lo que les permite soportar los gradientes de alta temperatura que se dan en los sistemas de HPLC. Mantiene su estabilidad dimensional y sus propiedades mecánicas en un amplio intervalo de temperaturas, lo que permite un funcionamiento eficaz en condiciones de HPLC analítica y preparativa.

  7. Facilidad de uso: El tubo PEEK es ligero y flexible, lo que facilita su manipulación, corte y conexión en un sistema de HPLC. Puede cortarse fácilmente a la longitud deseada y conectarse fácilmente a diversos componentes de HPLC, como válvulas de inyección, detectores y puertos de entrada/salida de columnas. La flexibilidad del tubo PEEK facilita la instalación y el mantenimiento de los sistemas HPLC.

  8. Compatibilidad: El tubo PEEK es compatible con una amplia gama de componentes de sistemas HPLC, incluidos accesorios, conectores y detectores. Puede integrarse fácilmente en configuraciones de HPLC existentes sin necesidad de grandes modificaciones o adaptadores.

En resumen, el tubo de PEEK es un material muy adecuado para aplicaciones de HPLC debido a su resistencia química, alta resistencia mecánica, bajo volumen muerto, inercia, biocompatibilidad, estabilidad térmica, facilidad de uso y compatibilidad con los componentes del sistema de HPLC. Garantiza un rendimiento fiable y constante, permitiendo separaciones precisas y reproducibles en diversas aplicaciones de HPLC analítica y preparativa.